Role of Yanghe Huayan Decoction in ER-PI3K/Akt/mTOR pathway of endocrine-resistant breast cancer cells with low mTOR expression

作     者：Xue Sun Xi-Xi Xu Xing-Yu Wang Run-Jia Luo Jing-Wei Li Xue Sun;Xi-Xi Xu;Xing-Yu Wang;Run-Jia Luo;Jing-Wei Li

作者机构：College of traditional Chinese medicineShandong University of traditional Chinese medicineJinanShandong250014 The first clinical college of Shandong University of traditional Chinese medicineJinanShandong250014 Department of breast and thyroid surgeryAffiliated Hospital of Shandong University of traditional Chinese medicineJinanShandong250014 

出 版 物：《Journal of Hainan Medical University》 (海南医学院学报（英文版）)

年 卷 期：2020年第26卷第14期

页      面：27-31页

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：National Natural Science Foundation of China(No.81573989) 

主　　题：Yanghe Huayan Decoction Breast cancer Endocrine resistance Low-level expression of Mtor ER-PI3K/Akt/mTOR 

摘      要：Objective:To study the mechanism of action of Yanghe Huayan Decoction on endocrine-resistant cells(MCF-7R/mTOR cells)with low expression of mTOR;Methods:CCK-8 assay and cell clone assay were used to detect cell proliferation and clonal ability,and flow cytometry was used to detect cell *** changes of cytokines in ER-PI3K/Akt/mTOR signaling pathway were analyzed by blot and ***:Yanghe Huayan Decoction could significantly affect the proliferation(p0.05)and cloning ability(p0.05)of MCF-7R/mTOR cells,promote cell apoptosis(p0.01),and downregulate the expression of ER,PI3K,AKT,mTOR and p-mTOR(p0.05).Conclusion:Yanghe Huayan Decoction can regulate the ER-PI3K/Akt/mTOR signaling pathway with multiple targets,and the use of combined mTOR inhibitors can regulate the ER-PI3K/Akt/mTOR signaling pathway more significantly.