Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

作     者：Qiang Wangn Xiaojun He Bin Wang Jun Pan Chunying Shi Jie Li Liudi Wang Yannan Zhao Jianwu Dai Dongjin Wang Qiang Wang;Xiaojun He;Bin Wang;Jun Pan;Chunying Shi;Jie Li;Liudi Wang;Yannan Zhao;Jianwu Dai;Dongjin Wang

作者机构：Department of Thoracic and Cardiovascular Surgerythe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Center for Clinical Stem Cell Researchthe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Human AnatomyHistology and EmbryologySchool of Basic MedicineQingdao UniversityQingdao 266021China Department of Cardiologythe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Key Laboratory of Molecular Developmental BiologyInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100190China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2021年第64卷第2期

页      面：269-281页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the Key Research Program of the Chinese Academy of Sciences(ZDRW-ZS-2016-2-2) the National Key Research and Development Program of China(2016YFC1000808) the National Natural Science Foundation of China(81370239) the Key Project supported by Medical Science and Technology Development Foundation,Nanjing Department of Health(201605016) the Key Project supported by Nanjing Medical Science and Technique Development Foundation(QRX17044) the Youth Innovation Promotion Association CAS Project(2016096) 

主　　题：injectable collagen scaffold mesenchymal stem cell MI 

摘      要：Stem cell therapy is an attractive approach for recovery from myocardial infarction(MI)but faces the challenges of rapid diffusion and poor survival after *** we developed an injectable collagen scaffold to promote the long-term retention of transplanted cells in chronic ***-five minipigs underwent left anterior descending artery(LAD)ligation and were equally divided into three groups 2 months later(collagen scaffold loading with human umbilical mesenchymal stem cell(hUMSC)group,hUMSC group,and placebo group(only phosphate-buffered saline(PBS)injection)).Immunofluorescence staining indicated that the retention of transplanted cells was promoted by the collagen *** and cardiac magnetic resonance imaging(CMR)showed much higher left ventricular ejection fraction(LVEF)and lower infarct size percentage in the collagen/hUMSC group than in the hUMSC and placebo groups at 12 months after *** were also higher densities of vWf-,α-sma-,and cTnT-positive cells in the infarct border zone in the collagen/cell group,as revealed by immunohistochemical analysis,suggesting better angiogenesis and more cardiomyocyte survival after ***,the injectable collagen scaffold was safe and effective on a large animal myocardial model,which is beneficial for constructing a favorable microenvironment for applying stem cells in clinical MI.