Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells作者机构：Department of Biochemistry Faculty of Veterinary Medicine Mansoura University Department of Biochemistry Faculty of Veterinary Medicine Kafrelsheikh University Urology and Nephrology Center Mansoura University Department of Pathology Faculty of Veterinary Medicine Kafrelsheikh University Department of Pathology Faculty of Veterinary Medicine Mansoura University
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2015年第5卷第5期
页 面：375-381页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis
摘 要：Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light ***: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes ***: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.
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