Chitosan hydrogel incorporated with dental pulp stem cell-derived exosomes alleviates periodontitis in mice via a macrophage-dependent mechanism

作     者：Zongshan Shen Shuhong Kuang Yong Zhang Mingmei Yang Wei Qin Xuetao Shi Zhengmei Lin 

作者机构：Hospital of StomatologyGuangdong Provincial Key Laboratory of StomatologyGuanghua School of StomatologySun Yat-sen UniversityGuangzhouGuangdongChina National Engineering Research Centre for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhouChina 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2020年第5卷第4期

页      面：1113-1126页

核心收录：

学科分类：081702[工学-化学工艺] 1003[医学-口腔医学] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant Nos.81873713,81670984 , 81700959) Science and Technology Program of Guangdong Province(2017B090911008,2016A030306018) the International Cooperation Project of Science and Technology in Guangdong Province(Grant No.2016B050502008) 

主　　题：Dental pulp stem cells Exosomes Chitosan hydrogel Periodontitis Macrophages 

摘      要：Periodontitis is caused by host immune-inflammatory response to bacterial insult.A high proportion of proinflammatory macrophages to anti-inflammatory macrophages leads to the pathogenesis of *** stem cell-derived exosomes can modulate macrophage phenotype,dental pulp stem cell-derived exosomes(DPSC-Exo)can effectively treat *** this study,we demonstrated that DPSC-Exo-incorporated chitosan hydrogel(DPSC-Exo/CS)can accelerate the healing of alveolar bone and the periodontal epithelium in mice with *** Ontology(GO)term enrichment analysis showed that treatment with DPSC-Exo/CS ameliorated periodontal lesion by suppressing periodontal inflammation and modulating the immune ***,DPSC-Exo/CS facilitated macrophages to convert from a pro-inflammatory phenotype to an anti-inflammatory phenotype in the periodontium of mice with periodontitis,the mechanism of which could be associated with miR-1246 in *** results not only shed light on the therapeutic mechanism of DPSCExo/CS but also provide the basis for developing an effective therapeutic approach for periodontitis.