Aspartic acid in the hippocampus:a biomarker for postoperative cognitive dysfunction

作     者：Rong Hu Dong Huang Jianbin Tong Qin Liao Zhonghua Hu Wen Ouyang 

作者机构：Department of AnesthesiaThird Xiangya HospitalCentral South University Department of Anatomy&NeurobiologyXiangya School of MedicineCentral South University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第2期

页      面：143-152页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China No.30871306 

主　　题：nerve regeneration brain impairment anesthesia postoperative cognitive dysfunction isoflurane hippocampal metabolites metabonomics gas chromatography-mass spectrometry aspar-tic acid NSFC grant neural regeneration 

摘      要：This study established an aged rat model of cognitive dysfunction using anesthesia with 2% iso- flurane and 80% oxygen for 2 hours. Twenty-four hours later, Y-maze test results showed that isoflurane significantly impaired cognitive function in aged rats. Gas chromatography-mass spectrometry results showed that isoflurane also significantly increased the levels of N,N-diethy- lacetamide, n-ethylacetamide, aspartic acid, malic acid and arabinonic acid in the hippocampus of isoflurane-treated rats. Moreover, aspartic acid, N,N-diethylacetamide, n-ethylacetamide and malic acid concentration was positively correlated with the degree of cognitive dysfunction in the isoflurane-treated rats. It is evident that hippocampal metabolite changes are involved in the formation of cognitive dysfunction after isoflurane anesthesia. To further verify these results, this study cultured hippocampal neurons in vitro, which were then treated with aspartic acid （100 μmol/L）. Results suggested that aspartic acid concentration in the hippocampus may be a biomarker for predicting the occurrence and disease progress of cognitive dysfunction.