Ursolic acid induces neural regeneration after sciatic nerve injury
Ursolic acid induces neural regeneration after sciatic nerve injury作者机构:Department of Hand SurgeryChina-Japan Union Hospital of Jilin University Department of NeurologyChina-Japan Union Hospital of Jilin University
出 版 物:《Neural Regeneration Research》 (中国神经再生研究(英文版))
年 卷 期:2013年第8卷第27期
页 面:2510-2519页
核心收录:
学科分类:1002[医学-临床医学] 100210[医学-外科学(含:普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金:financially sponsored by the Science and Technology Ministry of Jilin Province No.200705318
主 题:neural regeneration traditional Chinese medicine ursolic acid triterpenoid sciatic nerve peripheralnerve injury S100 muscular atrophy nerve myelin sheath grants-supported paper neuroregeneration
摘 要:In this study, we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve. BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuber-osity. The successful y generated model mice were treated with 10, 5, or 2.5 mg/kg ursolic acid via intraperitoneal injection. Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradual y increased at 1-4 weeks after sciatic nerve injury, and significantly decreased at 8 weeks. As such, ursolic acid has the capacity to significantly increase S100 protein expression levels. Real-time quantitative PCR showed that S100 mRNA expression in the L 4-6 segments on the injury side was increased after ursolic acid treatment. In addition, the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid. Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid. 10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid. Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice fol owing sciatic nerve injury.