Layer by layer surface engineering of poly (lactide-co-glycolide) nanoparticles:A versatile tool for nanoparticle engineering for targeted drug delivery

作     者：ROMERO Gabriela MURRAY Richard A. QIU Yuan SANZ David MOYA Sergio E. 

作者机构：Biosurfaces DepartmentCIC biomaGUNE Department of Polymer Science and EngineeringZhejiang University 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2013年第56卷第8期

页      面：1029-1039页

核心收录：

学科分类：100702[医学-药剂学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：funded by the Spanish Ministry of Science and Innovation(MAT2010-18995) the Marie Curie project "Transport Studies on Polymer Based Nanodevices and Assemblies for Delivery and Sensing"(TRASNADE) (- FP7 People International Research Staff Exchange Scheme Grant reference:247656) the Marie Curie Project "Design of Novel Polyelectrolyte Multilayer Based Delivery Systems for Therapeutic Antibodies and siRNA" (DeSIRNA) (FP7-People IAPP Grant reference 251646) 

主　　题：layer by layer PLGA NPs cell uptake antibody delivery lipid layers intracellular release 

摘      要：Recent work regarding the Layer by Layer （LbL） engineering of poly（lactide-co-glycolide） nanoparticles （PLGA NPs） is reviewed here. The LbL engineering of PLGA NPs is applied as a means of generating advanced drug delivery devices with tailored recognition, protection, cargo and release properties. LbL in combination with covalent chemistry is used to attach PEG and folic acid to control cell uptake and direct it towards cancer cells. LbL coatings composed of chitosan and alginate show low protein interactions and can be used as an alternative to Pegylation. The assembly on top of LbL coatings of lipid layers composed of variable percentages of 1,2-dioleoyl-sn-glycero-3-choline （DOPC） and 1,2-dioleoyl-sn-glycero-3-phospho- L-serine （DOPS） increases NP uptake and directs the NPs towards the endoplasmic reticulum. The antibody anti-TNF-ct is encapsulated forming a complex with alginate that is assembled LbL on top of PLGA NPs. The antibody is released in cell culture following first order kinetics. The release kinetics of encapsulated molecules inside PLGA NPs are studied when the PLGA NPs are coated via LbL with different polyelectrolytes. The intracellular release of encapsulated Doxorubicin is studied in the HepG2 cell line by means of Fluorescence Lifetime Imaging.