Betula platyphylla BpH0 X2 transcription factor binds to different cis_acting elements and confers osmotic tolerance

作     者：Zilong Tan Xuejing Wen Yucheng Wang Zilong Tan;Xuejing Wen;Yucheng Wang

作者机构：CAS Key Laboratory of Biogeography and Bioresource in Arid LandXinjiang Institute of Ecology and GeographyChinese Academy of^SciencesUrumqi 830011China State Key Laboratory of Desert and Oasis EcologyXinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China Graduate SchoolUniversity of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第11期

页      面：1762-1779页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：This work was supported by the National Natural Science Foundation of China(31770704) 

主　　题：markedly elements slightly 

摘      要：The homeodomain-leucine zipper(HD-Zip)proteins play crucial roles in plant developmental and environmental ***,how they mediate gene expression to facilitate abiotic stress tolerance remains *** the present study,we characterized BpHOX2(encoding a HD-Zip I family protein)from birch(Betula platyphylla).BpHOX2 is predominately expressed in mature stems and leaves,but expressed at a low level in apical buds and roots,suggesting that it has tissue-specific ***2 expression was highly induced by osmotic and salt,but only slightly induced by abscisic *** of BpHOX2 markedly improved osmotic tolerance,while knockdown of BpHOX2 increased sensitivity to osmotic ***2 could induce the expression of pyrroline-5-carboxylate synthase,peroxidase,and superoxide dismutase genes to improve proline levels and the reactive oxygen species scavenging *** immunoprecipitation sequencing combined with RNA sequencing showed that BpHOX2 could bind to at least four cis-acting elements,including dehydrationresponsive element“RCCGAC,Myb-p binding box“CCWACC,and two novel cis-acting elements with the sequences of“AAGAAGand“TACGTG(termed HBS1 and HBS2,respectively)to regulate gene *** results suggested that BpHOX2 is a transcription factor that binds to different cis-acting elements to regulate gene expression,ultimately improving osmotic tolerance in birch.