Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting

作     者：Han-Tso Lin Shih-Hwa Chiou Chung-Lan Kao Yi-Ming Shyr Chien-Jen Hsu Yin-Wen Tarng Larry L-T Ho Ching-Fai Kwok Hung-Hai Ku 

作者机构：Department of Medical Research and EducationTaipei Veterans General HospitalTaipei11217TaiwanChina Department of Medical Research and EducationTaipei Veterans General Hospitaland Institute of Clinical MedicineNational Yang-Ming UniversityTaipei11221TaiwanChina Department of Physical Medicine and RehabilitationTaipei Veterans General HospitalTaipei11217TaiwanChina Department of SurgeryTaipei Veterans General HospitalTaipei11217TaiwanChina Department of OrthopedicsKaohsiung Veterans General Hospital Kaohsiung81346TaiwanChina Division of Endocrinology and MetabolismDepartment of Internal MedicineTaipei Veterans General HospitalTaipei11217TaiwanChina Institute of Anatomy and Cell BiologyNational Yang-Ming UniversityTaipeiDepartment of Education and ResearchTaipei City Hospital11221TaiwanChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2006年第12卷第28期

页      面：4529-4535页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by National Science Council  Yen-Tj ing-Ling Medical Foundation and Taipei Veterans General Hospital 

主　　题：Putative pancreas stem cell Nestirr, pdx-1 Phenotypic marker 

摘      要：AIM： To isolate putative pancreatic stem cells （PSCs） from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cytometry. The potential for pancreatic lineage and the capability of β-cell differentiation in these PSCs were evaluated as well. METHODS： By using serum-free medium supplemented with essential growth factors, we attempted to isolate the putative PSCs which has been reported to express nestin and pdx-1. The MatrigelTM was employed to evaluate the differential capacity of isolated cells. Dithizone staining, insulin content/secretion measurement, and immunohistochemistry staining were used to monitor the differentiation. Fluorescence activated cell sorting （FACS） was used to detect the phenotypic markers of putative PSCs. RESULTS： A monolayer of spindle-like cells was cultivated. The putative PSCs expressed pdx-1 and nestin. They were also able to differentiate into insulin-, glucagon-, and somatostatin-positive cells. The spectrum of phenotypic markers in PSCs was investigated; a similarity was revealed when using human bone marrow-derived stem cells as the comparative experiment, such as CD29, CD44, CD49, CD50, CD51, CD62E, PDGFR-α, CD73 （SH2）, CD81, CD105（SH3）. CONCLUSION： In this study, we successfully isolated PSCs from adult human pancreatic duct by using serumfree medium. These PSCs not only expressed nestin and pdx-1 but also exhibited markers attributable to mesenchymal stem cells. Although work is needed to elucidate the role of these cells, the application of these PSCs might be therapeutic strategies for diabetes mellitus.