PERFORMANCE AND PERSISTENCE OF GREEN FLUORESCENT PROTEIN (gfp) MARKED AZOTOBACTER CHROOCOCCUM IN STERILIZED AND UNSTERILIZED WHEAT RHIZOSPHERIC SOIL

作     者：SINGH R KUMAR V SHARMA S BEHL RK SINGH BP NARULA N 

作者机构：Department of Microbiology SBS Post Graduate Institute of Biomedical Sciences & Research Balawala Dehradun India Department of Plant BreedingCCS Haryana Agricultural University Hisar India Department of AgronomyCCS Haryana Agricultural University Hisar India Department of Agronomy Microbiology CCS Haryana Agricultural University Hisar India 

出 版 物：《应用与环境生物学报》 (Chinese Journal of Applied and Environmental Biology)

年 卷 期：2005年第11卷第6期

页      面：751-755页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0901[农学-作物学] 0836[工学-生物工程] 0713[理学-生态学] 

主　　题：绿色荧光蛋白 固氮菌 消毒方法 小麦 

摘      要：The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref