Protective effect of selenium-enriched lactobacilluson CCI_4-induced liver injury in mice and its possible mechanisms

作     者：Long Chen Dao-Dong Pan Juan Zhou Ying-Zi Jiang 

作者机构：College of Life SciencesNanjing Normal University Jiangsu Engineering Research Center for Bio-Medical Function Materials Nanjing 210097 Jiangsu Province China Department of Food Sciences Nanjing Normal University Nanjing 110097 Jiangsu Province China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2005年第11卷第37期

页      面：5795-5800页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by the Special Programs of State Science and Technology Ministry of China During the 10~(th) 5-Year Plan Period  No. 2002BA518 A12  and Open Foundation from Key Laboratory of Resource Biotechnology of Jiangsu Province  China  No. KJS00033 

主　　题：Se-enriched lactobacillus Liver injury Carbon tetrachloride Macrophage Ca^2+ 

摘      要：AIM： To study the protective effects and mechanisms of Se-enriched lactobacillus on liver injury caused by carbon tetrachloride （CCl4） in mice. METHODS： Seventy-two ICR mice were randomly divided into four groups： normal group, CCl4-induced model group, low Se-enriched lactobacillus treatment group （L-Se group）, and high Se-enriched lactobacillus treatment group （H-Se group）. During a 3-wk experimental period, the common complete diet was orally provided daily for normal group and model group, and the mice in L-Se and H-Se groups were given a diet with 2 and 4 mg of organoselenium from Se-enriched lactobacillus per kg feed, respectively. From the 2nd wk of experiment, the model group, L-Se group, and H-Se group received abdominal cavity injection of olive oil solution containing 500 mL/L CCl4 （0.07 mL/100 g body mass） to induce liver injury, and the normal group was given olive oil on every other day for over 2 wk. In the first 2 wk post injection with CCl4, mice in each group were killed. The specimens of blood, liver tissue, and macrophages in abdominal cavity fluid were taken. Then the activities of the following liver tissue injury-associated enzymes including glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） as well as malondialdehyde （MDA） content were assayed. Changes of phagocytic rate and phagocytic index in macrophages were observed with Wright-Giemsa stain. Plasma TNF-α level was measured by radioimmunoassay. The level of intracellular free Ca^2＋ （[Ca^2＋]i） in hepatocytes was detected under a laser scanning confocal microscope. RESULTS： During the entire experimental period, the AST and ALT activities in liver were greatly enhanced by CCl4 and completely blunted by both low and high doses of Se-enriched lactobacillus. The Se-enriched lactobacillus- protected liver homogenate GSH-Px and SOD activities were higher or significantly higher than those in model group and were close t