Establishment of adult right ventricle failure in ovine using a graded, animal-specific pulmonary artery constriction model

作     者：Michael Nguyen-Truong Wenqiang Liu June Boon Brad Nelson Jeremiah Easley Eric Monnet Zhijie Wang Michael Nguyen-Truong;Wenqiang Liu;June Boon;Brad Nelson;Jeremiah Easley;Eric Monnet;Zhijie Wang

作者机构：School of Biomedical EngineeringColorado State UniversityFort CollinsCOUSA Veterinary Teaching HospitalColorado State UniversityFort CollinsCOUSA Department of Clinical SciencesColorado State UniversityFort CollinsCOUSA Department of Mechanical EngineeringColorado State UniversityFort CollinsCOUSA 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2020年第3卷第2期

页      面：182-192页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：animal models fibrosis pulmonary artery banding pulmonary hypertension right heart failure 

摘      要：Background: Right ventricle failure(RVF) is associated with serious cardiac and pulmonary diseases that contribute significantly to the morbidity and mortality of patients. Currently, the mechanisms of RVF are not fully understood and it is partly due to the lack of large animal models in adult RVF. In this study, we aim to establish a model of RVF in adult ovine and examine the structure and function relations in the ***: RV pressure overload was induced in adult male sheep by revised pulmonary artery constriction(PAC). Briefly, an adjustable hydraulic occluder was placed around the main pulmonary artery trunk. Then, repeated saline injection was performed at weeks 0, 1, and 4, where the amount of saline was determined in an animal-specific manner. Healthy, age-matched male sheep were used as additional controls. Echocardiography was performed bi-weekly and on week 11 post-PAC, hemodynamic and biological measurements were ***: This PAC methodology resulted in a marked increase in RV systolic pressure and decreases in stroke volume and tricuspid annular plane systolic excursion, indicating signs of RVF. Significant increases in RV chamber size, wall thickness, and Fulton s index were observed. Cardiomyocyte hypertrophy and collagen accumulation(particularly type III collagen) were evident, and these structural changes were correlated with RV ***: In summary, the animal-specific, repeated PAC provided a robust approach to induce adult RVF, and this ovine model will offer a useful tool to study the progression and treatment of adult RVF that is translatable to human diseases.