Recovery and Genetic Characterization of a West Nile Virus Isolate from China

作     者：Yan Guo Hongjiang Wang Songtao Xu Hangyu Zhou Chao Zhou Shihong Fu Mengli Cheng Fan Li Yongqiang Deng Xiaofeng Li Huanyu Wang Cheng-Feng Qin Yan Guo;Hongjiang Wang;Songtao Xu;Hangyu Zhou;Chao Zhou;Shihong Fu;Mengli Cheng;Fan Li;Yongqiang Deng;Xiaofeng Li;Huanyu Wang;Cheng-Feng Qin

作者机构：Department of VirologyState Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyAcademy of Military Medical Sciences(AMMS)Beijing 100071China PLA Strategic Support Force Characteristic Medical CenterBeijing 100071China Department of ArbovirusNHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious Disease Prevention and ControlChinese Center for Disease Control and PreventionBeijing 102206China Suzhou Institute of System MedicineChinese Academy of Medical Sciences and Peking Union Medical CollegeSuzhou 215000China Department of ImmunologyKey Laboratory of Immune Microenvironment and DiseaseNanjing Medical UniversityNanjing 211166China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第1期

页      面：113-121页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Key Research and Development Project of China(2016YFD0500304) the National Science and Technology Major Project of China(2018ZX09711003) the National Natural Science Foundation of China(81621005 and 31770190) supported by the National Science Fund for Distinguished Young Scholars(81925025) the Innovative Research Group(81621005)from the NSFC the Innovation Fund for Medical Sciences(2019-I2M-5–049)from the Chinese Academy of Medical Sciences 

主　　题：WNV isolates from China Phylogenetic analysis GA method Biological characteristic 

摘      要：West Nile virus(WNV)is an important neurotropic flavivirus that is widely distributed *** strain XJ11129 was first isolated in Xinjiang,China,and its genetic and biological characteristics remain largely *** this study,phylogenetic and sequence analyses revealed that XJ11129 belongs to lineage 1 a and shares high genetic identity with the highly pathogenic strain ***,the full-length genomic c DNA of XJ11129 was amplified and assembled using a modified Gibson assembly(GA)*** virus(named r XJ11129)was successfully rescued in days following this *** with other wild-type WNV isolates,r XJ11129 exhibited virulence indistinguishable from that of the NY99 strain in *** summary,the genomic and virulence phenotypes of r XJ11129 were characterized in vivo and in vitro,and these data will improve the understanding of the spread and pathogenesis of this reemerging virus.