Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs

作     者：Silvia de Oliveira Dorta Juliana Balbinotte Rose Monnerat Joao Roberto Spotti Lopes Tatiane da Cunha Odimar Zanuzo Zanardi Marcelo Pedreira de Miranda Marcos Antonio Machado Juliana de Freitas-Astua Sílvia de Oliveira Dorta;Juliana Balbinotte;Rose Monnerat;Joāo Roberto Spotti Lopes;Tatiane da Cunha;Odimar Zanuzo Zanardi;Marcelo Pedreira de Miranda;Marcos Antonio Machado;Juliana de Freitas-Astúa

作者机构：Programs de Pos-Graduagao em Microbiologia AgricolaEscola Superior de Agricultura Luiz de QueirozUniversidade de Sao PauloPiracicabaSPBrazil Laboratorio de BiotecnologiaCentro de Citricultura Sylvio MoreiraInstituto Agronomico de CampinasCordeiropolisSPBrazil Laboratorio de Insetos Vetores de FitopatdgenosDepartamento de Entomologia e AcarologiaEscola Superior de Agricultura Luiz de QueirozUniversidade de Sao PauloPiracicabaSPBrazil Embrapa Recursos Geneticos e BiotecnologiaBrasiliaDFBrazil Fundo de Defesa da Citricultura(Fundecitrus)AraraquaraSPBrazil Embrapa Mandioca e FruticulturaCruz das AlmasBABrazil Laboratdrio de Bioquimica FitopatoldgicaInstituto BioldgicoSao PauloSPBrazil 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第3期

页      面：519-530页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：This study was financed in part by the Coordenacao de Aperfeigoamento de Pessoal de Nivel Superior-Brasil(CAPES)and by the Brazilian Agricultural Research Corporation(EMBRAPA-grant number 03.13.03.006.00.00)。 

主　　题：Asian citrus psyllid Citrus sinensis Cry toxins endophytic Bt huanglongbing young shoots 

摘      要：Bacillus thuringiensis(Bt)toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops.The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap‐sucking insects,such as the Asian citrus psyllid Diaphorina citri Kuwayama(Hemiptera:Liviidae),a vector of“Candidatus Liberibacter spp.,associated with citrus huanglongbing(HLB).In this study,translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil‐drench,and their systemic pathogenicity to D.citri nymphs were investigated.The pathogenicity of three wild‐type Bt strains against D.citri third‐instar nymphs was demonstrated.Among the 10 recombinant strains tested(each of them harboring a single cry or cyt gene),3 can be highlighted,causing 42%–77%and 66%–90%nymphal mortality at 2 and 5 d after inoculation,respectively.The isolation of Bt cells from young citrus shoots and dead nymphs,and PCR performed with specific primers,confirmed the involvement of the Bt strains in the psyllid mortality.This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D.citri nymphs that fed on these soil‐drench inoculated seedlings.The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D.citri and the identified genes can be used for the production of transgenic Bt citrus.