Cardioprotective effect of Yiqi Huoxue granule through regulation of mitophagy after myocardial infarction in rats

作     者：Xi Chen Jiangong Wu Shuwen Guo Hanrong Mo Tianwei Zhan Fanghe Li Hui Wang Pengfei Feng Lulu Wei Xi Chen;Jiangong Wu;Shuwen Guo;Hanrong Mo;Tianwei Zhan;Fanghe Li;Hui Wang;Pengfei Feng;Lulu Wei

作者机构：School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing100029China Department of Traditional Chinese MedicinePeking University People’s HospitalBeijing100044China Fangshan Hospital Beijing University of Chinese MedicineBeijing102499China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2020年第7卷第2期

页      面：171-180页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(81473552) 

主　　题：Myocardial infarction Yiqi Huoxue granule Mitochondrial autophagy 

摘      要：Objective:To investigate the cardioprotective effect of Yiqi Huoxue granule(YQHXG)in the regulation of autophagy in rats induced with myocardial infarction(MI).Methods:An acute MI animal model was established by ligation of the left anterior descending branch of the coronary artery in Sprague-Dawley ***,20 rats received sham operation were classified into a control *** remaining 59 rats were randomly divided into MI model group(n=19),YQHXG group(n=20),and perindopril group(n=20).Relevant indicators on days 7 and 28 were observed in each *** ventricular function was determined by *** structure and morphology of mitochondria,and the number of autophagic vesicles,were observed by transmission electron *** mRNA and protein expression levels of LC3,FUNDC1,Beclin-1,and BNIP3 were examined in the tissue of the MI marginal ***:Compared with the MI model group,YQHXG showed obvious improvements in cardiac *** the microscopic morphology of the heart tissue,myocardial tissue damage attenuated,autophagic signs of autophagosomes and autolysosomes reduced,vacuolization in mitochondria mitigated,and mitochondria arranged in *** could reduce the degree of tissue lesion after MI and regulate the expression of autophagy-related molecules at different *** Day 7,YQHXG significantly downregulated the expression of Fundc1,Becn1,Bnip3 mRNA and reduced the levels of FUNDC1,Beclin-1,BNIP3,and LC3 B proteins expression(all P.001).On Day 28,YQHXG could upregulate the expression of Becn1,Fundc1 and Bnip3 mRNA and increased the levels of the corresponding proteins expression(all P.001).Besides,it also increased LC3 B protein expression level(P=.0344).Conclusion:YQHXG regulated the expression of mitochondrial autophagy-related factors in myocardial tissue and mitochondrial autophagic activity at different stages to protect the heart following MI.