Adipose tissue-derived mesenchymal stem cells and chitosan/poly(vinyl alcohol)nanofibrous scaffolds for cartilage tissue engineering

作     者：Ghada Nour-Eldeen Mazen Abdel-Rasheed Amira M.EL-Rafei Osama Azmy Gehan T.El-Bassyouni 

作者机构：Molecular Genetics and Enzymology DepartmentNational Research CentreDokkiCairo 12622Egypt Stem Cell Research groupMedical Research Centre of ExcellenceNational Research CentreCairo 12622Egypt Reproductive Health Research DepartmentNational Research Centre33 El-Buhouth StDokkiCairo 12622Egypt RefractoriesCeramics and Building Materials DepartmentNational Research CentreDokkiCairo 12622Egypt 

出 版 物：《Cell Regeneration》 (细胞再生（英文）)

年 卷 期：2020年第9卷第1期

页      面：62-73页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：This research was financially supported by National Research Centre Cairo Egypt(Project grant no.P11010170) 

主　　题：Adipose tissue Mesenchymal stem cells Nanofibrous scaffolds Osteoarthritis Cartilage tissue engineering 

摘      要：Osteoarthritis(OA)has been defined as a chronic inflammatory joint disease characterized by progressive articular cartilage *** growing interest in regenerative medicine,using cell therapy and tissue engineering,where cellular components in combination with engineered scaffolds and bioactive materials were used to induce functional tissue *** the present study,nanofibrous scaffold based on chitosan(CS)/poly(vinyl alcohol)(PVA)were used to develop biologically functionalized biomaterial to mimic the extracellular matrix,allowing the human adipose tissue derived mesenchymal stem cells(ADSCs)to proliferate and differentiate to chondrogenic *** morphology of the nanofibrous mat was examined using field emission scanning electron microscope(FE/SEM).The characteristic functional groups and the nature of the chemical bonds between atoms were evaluated using Fourier transform infrared spectroscopy(FTIR)*** of the seeded cells was morphologically evaluated by scanning electron microscopy and by flow cytometry for the expression of the stem cell surface *** differentiation potential was verified after chondrogenic induction by analyzing the expression of chondrogenic marker genes using real-time(RT PCR).Current study suggest significant potential for the use of ADSCs with the nanofibrous scaffolds in improving the osteoarthritis pathology.