Effects on the quality of frozen-thawed alpaca(Lama pacos)semen using two different cryoprotectants and extenders

作     者：AlexeiSantiani WilfredoHuanca RómuloSapana TeodosioHuanca NéstorSepúlveda RaúlSánchez 

作者机构：VeterinaryInstitute for Tropical and Highland ResearchFaculty of Veterinary MedicineUniversidad Nacional Mayor de San MarcosP.O.Box 03-5034Lima 41Perú OuimsachataExperimental StationNational Institute of Agrarian ResearchPunoPerú Reproductive Biotechnology CenterUniversidad de La FronteraP.O.Box 54-DTemucoChile 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2005年第7卷第3期

页      面：303-309,页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This research was supported by the National Science and Technology Council (CONCYTEC) of Perú,Veterinary Institute for Tropical and Highland Research Dirección de Investigación de la Universidad de La Frontera (Research Project DIUFRO 13020 

主　　题：cryopreservation glycerol ethylene glycol extenders alpaca spermatozoa 

摘      要：Aim： To evaluate two extenders and two cryoprotectant agents （CPA） for alpaca semen cryopreservation. Methods： Semen samples were obtained from four adult alpacas （Lama pacos） and frozen using extender Ⅰ （TRIS, citrate, egg yolk and glucose） or extender Ⅱ （skim milk, egg yolk and fructose）, each containing either glycerol （G） or ethylene glycol （EG） as CPA. Consequently, four groups were formed： 1） extender Ⅰ-G; 2） extender Ⅰ-EG; 3） extender Ⅱ-G; and 4） extender Ⅱ-EG. Semen was diluted in a two-step process： for cooling to 5 ℃ （extenders without CPA）, and for freezing （extenders with CPA）. Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results： When compared, the motility after thawing was higher （P 〈 0.05） in groups Ⅱ-EG （20.0 %±6.7 %） and Ⅱ-G （15.3 %±4.1%） than that in groups Ⅰ-G （4.0 %±1.1%） and Ⅰ-EG （1.0 %±1.4 %）. Viable spermatozoa with intact acrosomes in groups Ⅱ-EG （18.7 %±2.9 %） and Ⅱ-G （12.7 %±5.9 %） were higher than that in groups Ⅰ-G （5.7 %±1.5 %） and Ⅰ-EG （4.0 %±1.0 %）. Conclusion： The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders. （Asian J Androl 2005 Sep; 7： 303-309）