Correlation of CD105 and Vascular Endothelial Growth Factor in Laser-induced Choroidal Neovascularization in Rats

作     者：Jianfeng Xu Yusheng Wang Xiumei Yang Peng Zhang Lijun Chen 

作者机构：Department of Ophthalmology Institute of Ophthalmology of Chinese PLA X~ing HospitalFourth Military Medical University XUan 710032 China 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：2006年第22卷第3期

页      面：166-174,183页

学科分类：10[医学] 

基　　金：Supported by National Natural Science Foundation ( No.30371516)   the Scientific Research Foundation for the ReturnedOverseas Chinese Scholars  State Education Ministry  China ( 2004)   Natural Science Foundation of Shanxi Province ( No.2004C246) and Scientific and Technological Innovation Foundation of Xijing Hospital ( No.XJCX04M003) 

主　　题：CD105 VEGF Choroidal neovascularization Brown-Norway rat 

摘      要：Purpose: Choroidal neovascularization (CNV) plays an important role in pathogenesis of age-related macular degeneration (AMD), ocular histoplasmosis syndrome (OHS) and so on. However, mechanisms of CNV formation are not fully understood. The aim of this study is to investigate the correlation between expressions of CD105 and vascular endothelial growth factor (VEGF) in experimental laser-induced CNV in rats. Methods: CNV model was established by 532 nm laser photocoagulation in Brown-Norway rats. The expression of CD105 and VEGF in CNV was observed by immunohistochemistry at 3, 7, 14, 21, 28 and 56 days after laser photocoagulation. The image analysis was performed with the professional software of Image-Pro Plus. Results: Fluorescein angiography showed fluorescein leakage in CNV from days 7 to 56 after photocoagulation. VEGF expression was mainly observed in vascular endothelial cells, ganglion cells, inner nuclear layers and retinal pigment epithelial cells in normal retina and vascular endothelial cells in normal choroid of the rats. On day 3 after photocoagulation, VEGF began to express in laser-induced lesions. VEGF was strongly expressed in CNV after 7 days (P0.05). CD105 was initially presented in CNV at 7 days and obviously expressed at 14 days after photocoagulation (P0.05). There was notablely direct correlation between CD105-positive-microvessel density and positively semiquantitative scoring of VEGF in the CNV(r=0.989, P0.01). Conclusions: There is direct correlation between the expression of CD105 and VEGF in the laser-induced CNV in rat. It suggests that CD105 and VEGF might participate in the new blood vessel formation and promote the growth of CNV.