Molecular Detection of Sugarcane Ratoon Stunting Disease in Hainan Sugarcane-growing Areas
作者机构：Sugarcane Research CenterInstitute of Tropical BiotechnologyChinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources UtilizationMinistry of Agriculture and Rural AreasHaikou 571101China College of Life ScienceNanjing Agricultural UniversityNanjing 210095China
出 版 物：《Plant Diseases and Pests》 (植物病虫害研究（英文版）)
年 卷 期：2020年第11卷第1期
页 面：6-9页
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
基 金：Supported by National Natural Science Foundation of China(31771865) National Sugar Industry Technology System"Scientists for Prevention and Control of Sugarcane Virus Diseases"(CARS-170301)
主 题：Sugarcane Sugarcane ratoon stunting disease(RSD) Molecular detection Detection rate Hainan
摘 要：[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing *** research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.
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