Standing surface acoustic wave-assisted fabrication of patterned microstructures for enhancing cell migration

作     者：Yancheng Wang Yue Wang Deqing Mei Zongkai Yu Dai Xue Yancheng Wang;Yue Wang;Deqing Mei;Zongkai Yu;Dai Xue

作者机构：State Key Laboratory of Fluid Power and Mechatronic SystemsSchool of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Key Laboratory of Advanced Manufacturing Technology of Zhejiang ProvinceSchool of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Department of Aerospace and Mechanical EngineeringThe George Washington UniversityWashingtonDC 20052USA 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2020年第3卷第2期

页      面：87-97页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0836[工学-生物工程] 0811[工学-控制科学与工程] 

基　　金：The authors acknowledge the funding support from the National Natural Science Foundation of China(U1809220) Zhejiang Provincial Funds for Distinguished Young Scientists of China(LR19E050001) Open Fund Project of Zhijiang Laboratory(2019MC0AB02) Fund for Creative Research Groups of National Natural Science Foundation of China(51821093). 

主　　题：Standing surface acoustic waves(SSAWs) Micropatterned structure Dynamic cell culture Microfluidic device 

摘      要：Microfluidic device with patterned microstructures on the substrate surface was used to regulate cell adhesion,morphology,and functions in tissue engineering.We developed a microfluidic device which employing microscale patterned microstructures to achieve enhanced cell adhesion and migration.Biocompatible hydrogel substrates with micro-wavy and lattice-patterned microstructures were fabricated using standing surface acoustic waves and ultraviolet solidification.After seeding the L929 mouse fibroblast cells onto the patterned substrate of the microfluidic device,we determined that the viability and proliferation rate of cell migration can be greatly enhanced.Furthermore,L929 cells showed two types of gathering modes after 48 h of culturing.Cell growth was guided by the patterned substrate used in the microfluidic device and showed differences in the location distribution.Therefore,the developed microfluidic device with patterned microstructures can extend the application of in vitro cell culturing for future drug development and disease diagnosis.