Screening of the whole human cytochrome P450 complement(CYPome)with enzyme bag cocktails

作     者：Sangeeta Shrestha Sharma Shishir Sharma Matthias Bureik Sangeeta Shrestha Sharma;Shishir Sharma;Matthias Bureik

作者机构：School of Pharmaceutical Science and TechnologyHealth Sciences PlatformTianjin UniversityTianjin300072China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2020年第10卷第3期

页      面：271-276页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 0702[理学-物理学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Cytochrome P450 Drug metabolism Fission yeast Luminescence Proluciferin 

摘      要：We have previously introduced the use of permeabilized fission yeast cells(enzyme bags)that recombinantly express full-length CYPs for drug metabolism *** enzyme bags are cells with pores that function as enzymes in *** can easily be prepared without a need for ultracentrifugation and may be used in similar protocols as *** this study we report the preparation of enzyme bag cocktails that permit the testing of multiple CYPs in a single enzyme bag ***,we established a convenient testing scheme that permits a rapid screen of all human CYPs for activity towards any given candidate *** important aspect of this approach is the reduction of individual CYP test *** a cocktail containing many CYPs tests negative,it follows that all CYPs included in that cocktail need not be tested individually,thus saving time and *** new protocol was validated using two probe substrates.