From nature to nurture:Essence and methods to isolate robust methanotrophic bacteria

作     者：Haritha Meruvu Hui Wu Ziyue Jiao Liyan Wang Qiang Fei 

作者机构：School of Chemical Engineering and TechnologyXi'an Jiaotong UniversityXi'anShaanxiChina State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina Luoyang TMAXTREE Biotechnology Co.Ltd.LuoyangChina Shaanxi Key Laboratory of Energy Chemical Process IntensificationXi'an Jiaotong UniversityXi'anChina 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2020年第5卷第3期

页      面：173-178页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work is supported by the National Key R&D Program of China(2018YFA0901500) National Natural Science Foundation of China(21878241)and Open Funding Project of the State Key Laboratory of Bioreactor Engineering 

主　　题：Methanotrophs Isolation Culture medium High throughput Adaptive laboratory evolution 

摘      要：Methanotrophic bacteria are entities with innate biocatalytic potential to biofilter and oxidize methane into simpler compounds concomitantly conserving energy,which can contribute to copious industrial *** future and efficacy of such industrial applications relies upon acquiring and/or securing robust methanotrophs with taxonomic and phenotypic *** several dramatic advances,isolation of robust methanotrophs is still a long-way challenging task with several lacunae to be filled in *** with high tolerance to methane can be isolated and cultivated by mimicking natural environs,and adopting strategies like adaptive metabolic *** review summarizes existent and innovative methods for methanotrophic isolation and purification,and their respective applications.A comprehensive description of new insights shedding light upon how to isolate and concomitantly augment robust methanotrophic metabolism in an orchestrated fashion follows.