Alendronate loaded graphene oxide functionalized collagen sponge for the dual effects of osteogenesis and anti-osteoclastogenesis in osteoporotic rats

作     者：Yuyang Zeng Muran Zhou Lifeng Chen Huimin Fang Shaokai Liu Chuchao Zhou Jiaming Sun Zhenxing Wang 

作者机构：Department of Plastic SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Wuhan Clinical Research Center for Superficial Organ ReconstructionWuhan430022China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2020年第5卷第4期

页      面：859-870页

核心收录：

学科分类：081702[工学-化学工艺] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2019YFA0110500) the National Natural Science Foundation of China(81701922,81873941) 

主　　题：Osteoporosis Osteoporotic bone defect Graphene oxide Osteoclast Alendronate 

摘      要：Graphene Oxide(GO)-related hydrogels have been extensively studied in hard tissue repair,because GO can not only enhance the mechanical properties of polymers but also promote osteogenic differentiation of mesenchymal stem ***,simple GO-related hydrogels are not ideal for the repair of osteoporotic bone defects as the overactive osteoclasts in ***(Aln)is known to inhibit osteoclasts and may bind to GO through covalent ***,delivering Aln in GO-related hydrogels may be effective to repair osteoporotic bone ***,we developed a control-released system which is constructed by collagen(Col)-GO sponges loaded with Aln(Col-GO-Aln)for osteoporotic bone defect *** vitro,Col-GO-Aln sponges prolonged the release period of Aln,and the sponge containing 0.05%(w/v)GO released Aln faster than sponge with 0.2%***,tartrate-resistant acid phosphatase(TRAP)and F-actin staining demonstrated that Col-GO-Aln sponges effectively inhibited osteoclastogenesis of *** vivo,micro-CT scan showed that the volume of newborn bone in defect site by 0.05%GO sponge was nearly three times larger than that of other ***,the CT and histological examinations of rat femur proved that Col-GO-Aln sponges decreased the number of osteoclasts and suppressed the systemic bone loss in osteoporotic *** findings reveal that the application of GO as carriers of anti-osteoporosis drugs is a viable treatment for *** results also underscore the potential of GO-related hydrogels with Aln-releasing capacity for bone regeneration in osteoporosis.