SIZE EXCLUSION CHROMATOGRAPHIC COLUMN PACKED WITH REGENERATED CELLULOSE GELS

作     者：Guang Yang Li-na Zhang Xiao-peng Xiong Xiao-dong Cao Yong-liang Yang Department of Chemistry Wuhan University Wuhan 430072, China 

作者机构：武汉大学 湖北 武汉 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学（英文版）)

年 卷 期：2001年第19卷第4期

页      面：415-419页

核心收录：

学科分类：082903[工学-林产化学加工工程] 0817[工学-化学工程与技术] 08[工学] 0829[工学-林业工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：This work was supported by the National Natural Science Foundation of China (59773026 and 5933070) 

主　　题：cellulose packing silk fibroin preparative chromatography polysaccharides 

摘      要：Microporous regenerated cellulose gel particles were prepared by mixing cellulose cuoxam with silk fibroin as pore former, and the mean pore size and pore volume of the particles were 525 nm and 7.27 mt g(-1), respectively. A preparative size-exclusion chromatography (SEC) column (550 mm x 20 mm) packed with the cellulose gel particles was used for the fractionation of two polysaccharides Dextran 07 (M-W = 7.14 x 10(4), d = 1.7) and Dextran 50 (M-W = 50.5 x 10(4), d = 3.8) in water phase. The fractionation range of the stationary phase covered M, from 3 x 10(3) to 1.1 x 10(6). The daily throughput was 2.9 g for Dextran 07 (D07) and 4.3 g for Dextran 50 (D50) with a flow-rate of 1.5 mt min(-1). The fractions obtained by using the SEC were analyzed by an analytical SEC combined with laser light scattering (LLS), and the polydispersity indices of fractions for Dextran 07 and Dextran 50 were determined to be 1.34-1.57 and 1.53-3.36, respectively. The preparative SEC is a simple, rapid, and suitable means not only for the fractionation of polysaccharides in water but also for other polymers in organic solvents.