Adenomyosis-derived extracellular vesicles endow endometrial epithelial cells with an invasive phenotype through epithelial-mesenchymal transition

作     者：Dayong Chen Hai Qiao Yiting Wang Ling Zhou Na Yin Liaoqiong Fang Zhibiao Wang 

作者机构：State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and TechnologyCollege of Biomedical EngineeringChongqing Key Laboratory of Biomedical EngineeringChongqing Medical University40016PR China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2020年第7卷第4期

页      面：636-648页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Chongqing Basic Science and Frontier Technology Research Project, (cstc2017jcyjAX0432) Chongqing Postdoctoral Scientific Research Project, (Xm2017084) National Natural Science Foundation of China, NSFC, (11604034, 31571453, 81127901) National Natural Science Foundation of China, NSFC National Key Research and Development Program of China, NKRDPC, (2011CB707900, 20170412) National Key Research and Development Program of China, NKRDPC 

主　　题：Adenomyosis Epithelialmesenchymal transition Extracellular vesicles Primary endometrial epithelial cells Pathogenesis 

摘      要：Extracellular vesicles from highly metastatic tumor cells have been shown to mediate epithelial-mesenchymal transition(EMT)-related events in recipient *** endometrial epithelial cells,EMT processes are known to be involved in the development of *** aimed to investigate whether adenomyosis-derived extracellular vesicles(AMEVs)are able to induce an EMT process in endometrial epithelial *** this study,AMEVs were isolated from patients with adenomyosis and characterized by transmission electron microscopy,Western blot,and nanoparticle *** endometrial epithelial cells(EECs)were derived from normal endometrium tissues from patients with leiomyoma and co-cultured with AMEVs in *** uptake was examined by fluorescence confocal *** invasion of EECs was confirmed by Transwell ***,Western blot,and qRT-PCR were performed on EECs to illustrate the expression levels of cytokeratin 19,E-cadherin,vimentin,and zinc finger E-boxbinding homeobox 1(ZEB1).The results indicated that the cellular fluorescence intensity gradually increased after 48 h of co-culture,but decreased after 72 *** co-culturing with AMEVs for 72 h,EECs expressed significantly lower levels of cytokeratin 19 and E-cadherin,and significantly higher levels of vimentin and *** these results demonstrated that AMEVs induce an EMT process and enhance the invasion of *** changes may contribute to the pathogenesis and progression of adenomyosis.