SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites

作     者：Liwen Zhou Lisi Zheng Kaishun Hu Xin Wang Ruhua Zhang Yezi Zou Li Zhong Shang Wang Yuanzhong Wu Tiebang Kang Liwen Zhou;Lisi Zheng;Kaishun Hu;Xin Wang;Ruhua Zhang;Yezi Zou;Li Zhong;Shang Wang;Yuanzhong Wu;Tiebang Kang

作者机构：Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhou 510060China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene RegulationMedical Research CenterSun Yat-sen Memorial HospitalSun Yat‐sen UniversityGuangzhouChina 

出 版 物：《Signal Transduction and Targeted Therapy》 (信号转导与靶向治疗（英文）)

年 卷 期：2020年第5卷第1期

页      面：1516-1527页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by grants from the National Key Research and Development Program of China 2016YFA0500304 to T.K. the National Nature Science Foundation in China(NSFC)81772922 to Y.W.,81702890 to X.W.,and 81530081 and 31571395 to T.K. Guangdong Natural Science Foundation Team Project(2014A030312015 to T.K) the Sci-Tech Project Foundation of Guangzhou City(201607020038 to T.K.) the Natural Science Foundation of Guangdong Province(2016A030310218 to Y.W.). 

主　　题：SSB1 damage NBS1 

摘      要：Human single-stranded DNA-binding protein 1(hSSB1)is required for the efficient recruitment of the MRN complex to DNA doublestrand breaks and is essential for the maintenance of genome integrity.However,the mechanism by which hSSB1 recruits NBS1 remains elusive.Here,we determined that hSSB1 undergoes SUMOylation at both K79 and K94 under normal conditions and that this modification is dramatically enhanced in response to DNA damage.SUMOylation of hSSB1,which is specifically fine-tuned by PIAS2α,and SENP2,not only stabilizes the protein but also enhances the recruitment of NBS1 to DNA damage sites.Cells with defective hSSB1 SUMOylation are sensitive to ionizing radiation,and global inhibition of SUMOylation by either knocking out UBC9 or adding SUMOylation inhibitors significantly enhances the sensitivity of cancer cells to etoposide.Our findings reveal that SUMOylation,as a novel posttranslational modification of hSSB1,is critical for the functions of this protein,indicating that the use of SUMOylation inhibitors(e.g.,2-D08 and ML-792)may be a new strategy that would benefit cancer patients being treated with chemo-or radiotherapy.