Expression of p-PPARγ in the aging thoracic aorta of spontaneously hypertensive rat and inhibitory effect of rosiglitazone

作     者：Hai-Feng Yuan Xiao-Lin Niu Deng-Feng Gao Guang-Hua Hao An-Qi Song Jin Wei 

作者机构：Department of Encephalopathythe Second Affiliated Hospital of Xi'an Jiaotong University Department of Cardiologythe Second Affiliated Hospital of Xi'an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases of Ministry of EducationXi'an Jiaotong University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第12期

页      面：977-981页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by a grant from the National Natural Science Foundation of China(Grant No.81070219) 

主　　题：P-PPARγ Spontaneously hypertensive rat Thoracic aorta Rosiglitazone Aging 

摘      要：Objective:To investigate the expression of phosphorylated peroxisome proliferators-activated receptor y(p-PPARY) in the aging thoracic aorta of spontaneously hypertensive rat(SHR) and the inhibitory effect of rosiglitazone on the phosphorylation of ***:16,32 and 64 week-old Wistar-Kyoto rats(WKY) and SHR were randomly and respectively divided into WKY,SHR and SHR+rosiglitazone group(9 in each group).The rats in SHR+rosiglitazone group were treated with rosiglitazone(5 mg/kg,intragastrically) for 56 d,whereas normal saline was applied in WKY and SHR *** blood pressure(SBP)of rats was measured by tail cuff *** damage of thoracic aorta was analyzed using Hematoxylin-Eosin(HE) *** staining and western blot were performed to test the level of p-PPARY protein in the thoracic aorta arising from each ***:The SBP in 16,32 and 64 week-old SHR were significantly higher as compared with those in matched WKY rats(P0.05,respectively).HE staining showed increased content of smooth muscle cell,wrinkled lining endothelium and increased thickness of internal elastic lamina in the thoracic aorta of *** staining and western blot indicated that the levels of p-PPARY in the thoracic aorta arising from SHR were obviously higher than those in the thoracic aorta arising from WKY rats(P0.05,respectively).Importantly,the high SBP,histopathological abnormalities of the thoracic aorta and elevated p-PPARY expression were prominently abrogated by rosiglitazone treatment in SHR(P0.05,respectively).Furthermore,the SBP,histopathological abnormalities of the thoracic aorta and p-PPARY expression were positively correlated with age in SHR(P0.05,respectively).Conclusions:The PPARY phosphorylation was observed in the thoracic aorta of SHR and its expression was increased by the increase of ***,rosiglitazone inhibited the PPARY phosphorylation and suppressed vascular aging in SHR