The microRNA miR-184 regulates the CYP303A1 transcript level to control molting of Locusta migratoria
作者机构：Research Institute of Applied BiologyShanxi UniversityTaiyuanShanxiChina College of Life ScienceShanxi UniversityTaiyuanShanxiChina Department of EntomologyKansas State UniversityManhattanKSUSA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2021年第28卷第4期
页 面：941-951页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：This work was supported by National Natural Science Foundation of China(Grant No.31730074,31761133021,31802018,31320103921) the Program for Top Young Academic Leaders of Higher Learning Institutions of Shanxi Province(TYAL)2017,Key Research and Development Program of Shanxi Province(201803D221004-5) The Natural Science Foundation of Shanxi Province,China(201801D221247)
主 题：CYP303A1 gene regulation insect molting Locusta migratoria miR-184
摘 要：Cytochrome P450 monooxygenases(CYPs)play essential physiological functions in ***303A1 is highly conserved in insect species studied to date,and shows an indispensable role for adult eclosion in both Locusta migratoria and Drosophila ***,how CYP303A1 is regulated to control insect developmental processes remains *** this study,we discovered functional binding sites for miR-184 in the coding sequence of *** luciferase reporter assay showed that miR-184 could target LmCYP303A1 and regulate its expression in ***,overexpression of miR-184 through microinjection of agomir to locusts reduced the transcripts of LmCYP303A1 and led to abnormal molting,which is similar to the phenotype of silencing LmCYP303A1 by direct injection of dsLmCYP303A1 to ***,down-regulation of miR-184 by injection of antagomir increased the LmCYP303A1 transcript and caused molting *** findings suggested that miR-184 could target LmCYP303A1 to regulate the molting process in ***,which might be considered as a novel target for pest control.
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