Genome-wide analysis of NDR1/HIN1-like genes in pepper(Capsicum annuum L.)and functional characterization of CaNHL4 under biotic and abiotic stresses
作者机构：Laboratory of plant immunity and ecological control of plant diseaseCollege of Plant ProtectionSouthwest University400716 ChongqingChina Department of Botany and Plant BiologySection of BiologyFaculty of ScienceUniversity of Geneva1211 Geneva 4Switzerland State Key Laboratory of North China Crop Improvement and RegulationCollege of Plant ProtectionHebei Agricultural University071001 BaodingChina
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2020年第7卷第1期
页 面：1611-1625页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金：supported by The National Natural Science Foundation of China(31670148,31870147) the Fundamental Research Funds for the Central Universities(XDJK2020B064) the Science and Technology Projects of the Chongqing Company of China Tobacco Corporation(NY20180401070001,NY20180401070008,NY20180401070010) Southwest University’s Training Program of Innovation and Entrepreneurship for Undergraduates(Project No.X201910635133)
主 题：pepper analysis functional
摘 要：Plant NDR1/HIN1-like(NHL)genes play an important role in triggering plant defenses in response to biotic *** this study,we performed a genome-wide identification of the NHL genes in pepper(Capsicum annuum L.)and characterized the functional roles of these CaNHL genes in response to abiotic stresses and infection by different *** analysis revealed that CaNHLs can be classified into five distinct subgroups,with each group containing generic and specific *** element analysis showed that the majority of the promoter regions of the identified CaNHLs contain jasmonic acid(JA)-responsive and salicylic acid(SA)-responsive elements,and transcriptomic analysis revealed that CaNHL genes are expressed in all the examined tissues of *** CaNHL1,CaNHL4,CaNHL6,CaNHL10,CaNHL11,and CaNHL12 genes were significantly upregulated under abiotic stress as well as in response to different pathogens,such as TMV,Phytophthora capsici and Pseudomonas *** addition,we found that CaNHL4 localizes to the plasma ***4-silenced pepper plants display significantly increased susceptibility to TMV,Phytophthora capsici and Pseudomonas syringae,exhibiting reduced expression of JA-related and SA-related genes and reduced ROS ***,transient overexpression of CaNHL4 in pepper increases the expression of JArelated and SA-related genes,enhances the accumulation of ROS,and inhibits the infection of these three ***,for the first time,we identified the NHL genes in pepper and demonstrated that CaNHL4 is involved in the production of ROS and that it also regulates the expression of JA-related and SA-related genes in response to different pathogens,suggesting that members of the CaNHL family play an essential role in the disease resistance of pepper.
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