Monitoring Neutralization Property Change of Evolving Hantaan and Seoul Viruses with a Novel Pseudovirus-Based Assay

作     者：Tingting Ning Ling Wang Shuo Liu Jian Ma Jianhui Nie Weijin Huang Xuguang Li Yuhua Li Youchun Wang Tingting Ning;Ling Wang;Shuo Liu;Jian Ma;Jianhui Nie;Weijin Huang;Xuguang Li;Yuhua Li;Youchun Wang

作者机构：Division of HIV/AIDS and Sexually Transmitted Virus VaccinesNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China Division of Arboviruses VaccineNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China Centre for Biologics EvaluationBiologics and Genetic Therapies DirectorateHealth CanadaOttawaON K2J 4X9Canada 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第1期

页      面：104-112页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Science and Technology Major Projects of Drug Discovery[Grant Number 2018ZX09101-001] 

主　　题：Hemorrhagic fever with renal syndrome(HFRS) Hantaan virus(HTNV) Seoul virus(SEOV) Pseudovirus-based neutralization assay(PBNA) Amino acid substitution Vaccine 

摘      要：The Hantaan virus(HTNV)and Seoul virus(SEOV)mutants have accumulated over *** is important to determine whether their neutralizing epitopes have evolved,thereby making the current vaccine ***,it is impossible to determine by using traditional plaque reduction neutralization test(PRNT),because it requires large numbers of live mutant ***-based neutralization assays(PBNA)were developed by employing vesicular stomatitis virus(VSV)backbone incorporated with HTNV or SEOV glycoproteins(VSVDG*-HTNVG or VSVDG*-SEOVG).56 and 51 single amino acid substitutions of glycoprotein(GP)in HTNV and SEOV were selected and introduced into the reference *** the mutant pseudoviruses were generated and tested by *** PBNA results were highly correlated with PRNT ones with R2 being 0.91 for VSVDG*-HTNVG and 0.82 for VSVDG*-SEOVG.53 HTNV mutant pseudoviruses and 46 SEOV mutants were successfully ***,by using PBNA,we found that HTNV or SEOV immunized antisera could neutralize all the corresponding 53 HTNV mutants or the 46 SEOV mutants *** novel PBNA enables us to closely monitor the effectiveness of vaccines against large numbers of evolving HTNV and *** the current vaccine remains to be effective for the naturally occurring mutants.