Kinetic Determination of Uric Acid in Human Serum by Using the Uncatalyzed BZ Reaction in Non-equilibrium Steady State

作     者：Ren,Jie Yang,Wu Niu,Xiuli Gao,Jinzhang 

作者机构：Chemistry&Chemical Engineering CollegeNorthwest Normal UniversityLanzhouGansu 730070China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2012年第30卷第2期

页      面：288-292页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0817[工学-化学工程与技术] 0828[工学-农业工程] 0804[工学-仪器科学与技术] 081701[工学-化学工程] 

基　　金：This work was supported in part by the Project of International Cooperation between China and Ukraine (No. 043-05)  the National Natural Science Foundation of China (No. 20873101)  the Natural Science Foundation of Gansu (No. 1010RJZA015) and the project LKQN-08-9 of Northwest Normal University  China 

主　　题：non-equilibrium steady state uric acid uncatalyzed BZ reaction 

摘      要：A novel kinetic method for determination of uric acid in human serum by means of an uncatalyzed BZ system consisting of potassium bromate and p-hydroxybenzaldehyde （p-HBA） in sulfuric acid medium was proposed, in which the analyte perturbation to the system was recorded close to the bifurcation point. The potential change was directly proportional to the logarithm of concentration of uric acid in the range of 3.73 × 10 8--7.48 × 10-4 mol.L-1 （r=0.9983） with a detection limit of 7.45 × 10-9mol.L-I and a recovery from 98.9% to 101.1%. A comparison between the proposed technique and other methods indicated that results obtained were in agreement with those in clinical detection. In addition, the possible mechanism of action of uric acid on the uncatalyzed BZ reaction was also discussed briefly.