成年MECP2倍增小鼠中社交障碍相关神经集合的实时成像及功能挽救

作     者：孙乐 陈睿国 李龙 袁博 宋坤 潘娜 程田林 常世阳 林坤章 何晓斌 吴倩 徐富强 仇子龙 王晓群 Le Sun;Ruiguo Chen;Long Li;Bo Yuan;Kun Song;Na Pan;Tian-Lin Cheng;Shiyang Chang;Kunzhang Lin;Xiaobin He;Qian Wu;Fuqiang Xu;Zilong Qiu;Xiaoqun Wang

作者机构：State Key Laboratory of Brain and Cognitive ScienceCAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of Brain-Intelligence Technology(Shanghai)Institute for Stem Cell and RegenerationInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Institute of NeuroscienceCAS Key Laboratory of Primate NeurobiologyState Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China The College of Life ScienceUniversity of Chinese Academy of SciencesBeijing 100049China The College of Life ScienceHebei Normal UniversityShijiazhuang 050016China State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsWuhan Institute of Physics and MathematicsCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesWuhan 430071China Advanced Innovation Center for Human Brain ProtectionBeijing Institute for Brain DisordersCapital Medical UniversityBeijing 100069China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2020年第65卷第14期

页      面：1192-1202,M0004页

核心收录：

学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：This work was supported by the National Basic Research Program of China(2017YFA0103303) Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32010100,XDB02050400,XDB02050005,XDA16020601) National Basic Research Program of China(2017YFA0102601,2019YFA0110100) National Natural Science Foundation of China(NSFC)(91732301,31671072,31771140,81891001,91432111,81527901,31400977,31625013) Grants of Beijing Brain Initiative of Beijing Municipal Science&Technology Commission(Z181100001518004). 

主　　题：社交障碍 自闭症 神经活动 神经环路 社交行为 实时成像 CA1区 转基因小鼠 

摘      要：人类男性中MECP2基因的倍增将导致严重的自闭症症状.过表达人源MECP2基因,会使转基因小鼠(MECP2-TG)表现出类似自闭症的行为,但这种社交行为缺陷相关的神经环路机制尚不明确.本文在小鼠海马体CA1区植入微型荧光透镜,观察MECP2-TG小鼠和野生型小鼠的社交过程及相关的实时神经活动钙荧光信号,发现与社交行为紧密相关的神经活动在MECP2-TG小鼠中被削弱了.当使用CRISPR/Cas9技术,敲除成年MECP2-TG小鼠CA1区细胞中的过表达人源MECP2基因后,小鼠的社交障碍被缓解.这一数据提示了小鼠海马体中与社交行为相关的神经环路,并提出一种缓解成年自闭症患者社交障碍的潜在方法.