Modulation of TGFβ_2 and dopamine by PKC in retinal Müller cells of guinea pig myopic eye

作     者：Jun-Feng Mao, Shuang-Zhen Liu, Wen-Juan Qin, Qian Xiang Department of Ophthalmology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China 

作者机构：Department of Ophthalmology Xiangya Hospital of Central South University Changsha 410008 Hunan Province China 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2011年第4卷第4期

页      面：357-360页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：National Natural Science Foundation of China(No. 30600694) 

主　　题：protein kinase C retinal Müller cell transforming growth factor-β2 dopamine myopia 

摘      要：AIM: To investigate the effect of protein kinase C (PKC) on transforming growth factor-β2 (TGFβ2) and dopamine in retinal Müller cells of guinea pig myopic eye. METHODS: Myopia was induced by translucent goggles in guinea pig, whose retinal Müller cells were cultured using the enzyme-digesting method. Retinal Müller cells were divided into 5 groups: normal control, myopia, myopia plus GF109203X, myopia plus PMA, myopia plus DMSO. PKC activities were detected by the non-radioactive methods. TGFβ2 and tyrosine hydroxylase (TH) proteins were analyzed by Western Blotting in retinal Müller cells. Dopamine was determined by the high-performance liquid chromatography- electrochemical detection in suspensions. RESULTS: After 14 days deprived, the occluded eyes became myopic with ocular axle elongating. Müller cells of guinea pigs were obtained using enzyme digestion. Compared with normal control group, the increase in PKC activity and the up-regulation in TGFβ2 expression were found in retinal Müller cells of myopic eyes, with the decrease of TH and dopamine content (P 0.05). After PKC activated by PMA, TGFβ2 and TH content were up-regulated with the increase of dopamine content (P 0.05). While the PKC activities was inhibited by GF109203X, proteins of TGFβ2 and TH were down-regulated in the myopic eyes, with the decrease of dopamine content (P 0.05). CONCLUSION: TGFβ2 and dopamine are modulated by PKC in Müller cells of the myopic eyes in guinea pig.