Biosynthesis of Unsaturated Fatty Acids via Mortierella Isabellina Cultivated in a Medium Containing Butanol

作     者：XIAN Mo YAN Ji chang CHENG Tie xin KANG Yi jian LI Wen xing BI Ying li ZHEN Kai ji 

作者机构：College of ChemistryJilin UniversityChangchun 130023P.R.China College of ChemistryNortheast Normal UniversityChangchun 130024P.R.China Department of Chemical EngineeringQingdao Chimical Industry InstituteQingdao 266042P.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究(英文版))

年 卷 期：2002年第18卷第3期

页      面：303-306页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China(No.2 98730 18) 

主　　题：Mortierella isabellina Butanol Unsaturated fatty acid Biosynthesis 

摘      要：Mortierella isabellina was found to accumulate large amounts of unsaturated fatty acids when it was grown in a medium containing butanol(5 g/L) and yeast extract(10 g/L) and cultivated at 25 ℃ for 5 d during which additional feeding butanol of 2 g/L was fed after being cultivated for 48 h . The resultant mycelial lipids accounted for 40 1% of the dry mycelia, while about 34% of butanol in the medium was converted. The mycelial lipids contained four kinds of unsaturated fatty acids, i.e ., palmitoleic(4 9%), oleic(54 1%), linoleic(10 4%) and linolenic(5 4%) acids. Those accounted for 74 8% of the total fatty acids. The effects of the culture conditions, such as cultivation temperature, initial pH of the medium and additional feeding butanol in the course of cultivation, on the production of mycelial lipids by M. isabellina were studied.