Intraoperative single administration of neutrophil peptide 1 accelerates the early functional recovery of peripheral nerves after crush injury

作     者：Yu-Song Yuan Su-Ping Niu Fei Yu Ya-Jun Zhang Na Han Hao Lu Xiao-Feng Yin Hai-Lin Xu Yu-Hui Kou 

作者机构：Department of Trauma and OrthopedicsPeking University People’s HospitalPeking UniversityBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University)Ministry of EducationBeijingChina Office of Academic ResearchPeking University People’s HospitalBeijingChina National and Local Joint Engineering Research Center of Orthopaedic BiomaterialsDepartment of Bone&Joint SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinceChina National Center for Trauma MedicineBeijingChina Diabetic Foot Treatment CenterPeking University People’s HospitalPeking UniversityBeijingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第11期

页      面：2108-2115页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：funded by the National Natural Science Foundation of China,No.31571236(to YHK) the Key Laboratory of Trauma and Neural Regeneration(Peking University),Ministry of Education,China,No.BMU2019XY007-01(to YHK) the Ministry of Education Innovation Program of China,No.IRT_16R01(to YHK) the Research and Development Funds of Peking University People’s Hospital,China,Nos.RDH2017-01(to YHK),RDY2018-09(to HL)。 

主　　题：crush injury defensin 1 gait analysis intraoperative administration nerve conduction velocity nervous system neutrophil peptide 1 peripheral nerve injury peripheral nerve regeneration sciatic nerve tibialis anterior muscle trauma 

摘      要：Neutrophil peptide 1 belongs to a family of peptides involved in innate immunity. Continuous intramuscular injection of neutrophil peptide 1 can promote the regeneration of peripheral nerves, but clinical application in this manner is not convenient. To this end, the effects of a single intraoperative administration of neutrophil peptide 1 on peripheral nerve regeneration were experimentally observed. A rat model of sciatic nerve crush injury was established using the clamp method. After model establishment, a normal saline group and a neutrophil peptide 1 group were injected with a single dose of normal saline or 10 μg/mL neutrophil peptide 1, respectively. A sham group, without sciatic nerve crush was also prepared as a control. Sciatic nerve function tests, neuroelectrophysiological tests, and hematoxylin-eosin staining showed that the nerve conduction velocity, sciatic functional index, and tibialis anterior muscle fiber cross-sectional area were better in the neutrophil peptide 1 group than in the normal saline group at 4 weeks after surgery. At 4 and 8 weeks after surgery, there were no differences in the wet weight of the tibialis anterior muscle between the neutrophil peptide 1 and saline groups. Histological staining of the sciatic nerve showed no significant differences in the number of myelinated nerve fibers or the axon cross-sectional area between the neutrophil peptide 1 and normal saline groups. The above data confirmed that a single dose of neutrophil peptide 1 during surgery can promote the recovery of neurological function 4 weeks after sciatic nerve injury. All the experiments were approved by the Medical Ethics Committee of Peking University People s Hospital, China(approval No. 2015-50) on December 9, 2015.