Effect of dietary L-glutamate levels on growth,digestive and absorptive capability,and intestinal physical barrier function in Jian carp(Cyprinus carpio ***)

作     者：Ye Zhao Tian-Ran Zhang Qian Li Lin Feng Yang Liu Wei-Dan Jiang Pei Wu Juan Zhao Xiao-Qiu Zhou Jun Jiang Ye Zhao;Tian-Ran Zhang;Qian Li;Lin Feng;Yang Liu;Wei-Dan Jiang;Pei Wu;Juan Zhao;Xiao-Qiu Zhou;Jun Jiang

作者机构：College of Animal Science and TechnologySichuan Agricultural UniversityChengdu611130China Animal Nutrition InstituteSichuan Agricultural UniversityChengdu611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityYa'an625014China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2020年第6卷第2期

页      面：198-209页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：financially supported by National Key R&D Program of China(2019YFD0900200) National Natural Science Foundation of China(31702362) 

主　　题：L-glutamate Growth Digestive ability Intestinal barrier function Jian carp 

摘      要：The present study explored effects of L-glutamate(Glu)levels on growth,digestive and absorptive capability,and intestinal physical barrier functions of Jian carp(Cyprinus carpio).A total of 600 Jian carp(126.40±0.21 g)were randomly distributed into 5 groups with 3 replicates each,fed diets containing graded levels of Glu(53.4[control],57.2,60.6,68.4,and 83.4 g/kg)for 63 *** showed compared with control diet,feed intake and percent weight gain(PWG)in fish fed 83.4 g of Glu/kg diet were increased and feed conversion ratio in fish fed 68.4 g of Glu/kg diet was decreased(P0.05).Similarly,body crude protein and lipid contents in fish fed 68.4 g of Glu/kg diet were higher(P0.05).The activities of trypsin and chymotrypsin in the hepatopancreas and intestine,and amylase,alkaline phosphatase(AKP),Na^+,K^+-ATPase(NKA),and creatine kinase(CK)in intestine were higher in fish fed 68.4 g of Glu/kg diet(P0.05).Dietary Glu(57.2 to 83.4 g/kg diet)decreased malondialdehyde(MDA)and protein carbonyl(PCO)contents in the intestine(P0.05).The activities of catalase(CAT),glutathione peroxidase(GPx),and glutathione S-transferase(GST)in the hepatopancreas and intestine were higher in fish fed 60.6 and 68.4 g of Glu/kg diets(P0.05).Intestinal the glutathione reductase(GR)activity and glutathione(GSH)content in fish fed 60.6,68.4,and 83.4 g of Glu/kg diet were increased(P0.05).The GPx1 a,GST,and nuclear factor erythroid 2-related factor 2(Nrf2)mRNA expressions in the intestine were up-regulated in fish fed 60.6 and 68.4 g of Glu/kg diet(P0.05).The zonula occludens protein-1(ZO-1),occludin1,and claudin3 mRNA expressions were also up-regulated in fish fed 83.4 g of Glu/kg diet(P0.05).Fish fed 68.4 g of Glu/kg diet had higher levels of claudin 2,claudin7,and protein kinase C(PKC)mRNA(P0.05).These results indicated that Glu improved frsh growth,digestive and absorptive ability,and intestinal physical barrier *** on the quadratic regression analysis of PWG,and MDA of the