Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defence assays and correlations with anthropometric and laboratory parameters

作     者：Mihnea-Alexandru Găman Mirela Elena Epîngeac Camelia Cristina Diaconu Amelia Maria Găman 

作者机构："Carol Davila"University of Medicine and PharmacyBucharest 050474Romania Department of HematologyCenter of Hematology and Bone Marrow TransplantationFundeni Clinical InstituteBucharest 022328Romania Department of PathophysiologyUniversity of Medicine and Pharmacy of CraiovaCraiova 200349Romania Internal Medicine ClinicClinical Emergency Hospital of BucharestBucharest 014461Romania Clinic of HematologyFilantropia City HospitalCraiova 200143Romania 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2020年第11卷第5期

页      面：193-201页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Oxidative stress Obesity Diabetes Reactive oxygen species Antioxidants Dyslipidaemia 

摘      要：BACKGROUND Obesity and diabetes are associated with high levels of oxidative *** Romanian patients with obesity and(or)diabetes,this association has not been sufficiently *** To evaluate oxidative stress in obese and(or)diabetic subjects and to investigate the possible correlations between oxidative stress and anthropometric/biochemical *** Oxidative stress was evaluated from a single drop of capillary *** oxygen species(ROS)were evaluated using the free oxygen radical test(FORT).The free oxygen radical defence(FORD)assay was used to measure antioxidant *** FORT levels were higher in obese subjects(3.04±0.36 mmol/L H2O2)vs controls(2.03±0.14 mmol/L H2O2)(P0.0001).FORD levels were lower in obese subjects(1.27±0.13 mmol/L Trolox)vs controls(1.87±1.20 mmol/L Trolox)(P=0.0072).Obese diabetic subjects had higher FORT values(3.16±0.39 mmol/L H2O2)vs non-diabetic counterparts(2.99±0.33 mmol/L H2O2)(P=0.0233).In obese subjects,FORT values correlated positively with body mass index(BMI)(r=0.48,P=0.0000),waist circumference(WC)(r=0.31,P=0.0018),fasting plasma glucose(FPG)(r=0.31,P=0.0017),total cholesterol(TC)(r=0.27,P=0.0068)and uric acid(r=0.36,P=0.0001).FORD values correlated negatively with BMI(r=-0.43,P=0.00001),WC(r=-0.28,P=0.0049),FPG(r=-0.25,P=0.0130),TC(r=-0.23,P=0.0198)and uric acid(r=-0.35,P=0.0002).In obese diabetic subjects,FORT values correlated positively with BMI(r=0.49,P=0.0034)and TC(r=0.54,P=0.0217).FORD values were negatively associated with BMI(r=-0.54,P=0.0217)and TC(r=-0.58,P=0.0121).CONCLUSION Oxidative stress levels,as measured by the FORT and FORD assays,were higher in obese subjects vs *** levels were elevated in diabetic obese patients vs obese non-diabetic patients and controls.