A Salmonella enterica serovar Typhi plasmid induces rapid and massive apoptosis in infected macrophages

作     者：Shuyan Wu Yuanyuan Li Yang Xu Qiong Li Yuanyuan Chu Rui Huang Zhenghong Qin 

作者机构：Medical College of Soochow UniversitySuzhouChina 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2010年第7卷第4期

页      面：271-278页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Natural Science Foundation of China(No.30972768) the Special and General Postdoctoral Science Foundation of China(No.200902529 and No.20080430178) the Natural Science Foundation of Jiangsu High Education Institute of China(No.08KJB310009) the Social Development Science Foundation of Suzhou City of China(No.SS08025) 

主　　题：apoptosis macrophage plasmid Salmonella enterica serovar Typ 

摘      要：pRST98 is a chimeric plasmid isolated from Salmonella enterica serovar Typhi(***)that mediates the functions of drug resistance and ***,we reported that Salmonella plasmid virulence(spv)genes were present in *** our current study,we investigated whether plasmid pRST98 exhibits significant cytotoxicity in ***98 was transferred into the avirulent Salmonella enterica serovar Typhimurium(***)strain RIA to create the transconjugant pRST98/*** standard *** virulent strain SR-11,which carries a 100-kb virulence plasmid,was used as a positive *** bacterial strains were incubated with a murine macrophage-like cell line(J774A.1)in *** of J774A.1 cells was examined by electron microscopy and flow cytometry after annexin-V/propidium iodide labeling,and the survival of Salmonella strains in J774A.1 cells was *** showed that macrophages infected with strain pRST98/RIA displayed greater levels of apoptosis than those infected with RIA and that pRST98 may increase bacterial survival in *** studies showed that the pRST98-induced death of macrophages was associated with the loss of mitochondrial membrane potential and that pRST98 may activate caspase-9 and then *** research data indicate that the virulence of bacteria that contain the pRST98 plasmid is enhanced;the presence of this plasmid increases the survival of the bacterial pathogen and acts through the mitochondrial pathway to mediate macrophage apoptosis.