Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats

作     者：Erica Martins Ferreira Gotardo Gilberto de Almeida Ribeiro Thayane Rodrigues Leite Clemente Camila Henrique Moscato Renata Bortolin Guerra Tome Thalita Rocha Jose Pedrazzoli Jr Marcelo Lima Ribeiro Alessandra Gambero 

作者机构：Clinical Pharmacology and Gastroenterology UnitSao Francisco University Medical SchoolBraganca PaulistaSP 12916-900Brazil 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2014年第20卷第15期

页      面：4345-4352页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：The Fundacao de Amparo a Pesquisa do Estado de Sao Paulo No. FAPESP 2010/02991-6 

主　　题：Hepcidin 25, Interleukin 6, Iron, Stat 3, Anemia, Toll like receptor 4 

摘      要：AIM:To investigate hepcidin expression,interleukin-6(IL-6)production and iron levels in the rat colon in the presence of trinitrobenzene sulfonic acid(TNBS)-induced ***:In rats,we evaluated the severity of colitis induced by repeated TNBS administration using macroscopic and microscopic scoring systems and myeloperoxidase activity *** colonic levels of hepcidin,tumor necrosis factor alpha(TNF-α),IL-10 and IL-6 were measured by Enzyme-Linked Immunosorbent Assay,and hepcidin-25 expression and iron deposition were analyzed by immunohistochemistry and the Prussian blue reaction,***-3 phosphorylation was assessed by Western blot *** parameters,iron and transferrin levels,and transferrin saturation were also ***,the ability of iron,pathogen-derived molecules and IL-6 to induce hepcidin expression in HT-29 cells was ***:Repeated TNBS administration to rats resulted in macroscopically and microscopically detectable colon lesions and elevated colonic myeloperoxidase ***-25 protein levels were increased in colonic surface epithelia in colitic rats(10.2±4.0pg/mg protein vs 71.0±8.4 pg/mg protein,P0.01).Elevated IL-6 levels(8.2±1.7 pg/mg protein vs 14.7±0.7 pg/mg protein,P0.05),TNF-αlevels(1.8±1.2pg/mg protein vs 7.4±2.1 pg/mg protein,P0.05)and Stat-3 phosphorylation were also *** alterations in iron homeostasis,hepcidin levels and anemia were not detected in colitic *** deposition in the colon was only observed during *** gene expression was increased in HT-29 cells after IL-6 and lipopolysaccharide[a toll-like receptor 4(TLR-4)ligand]***,ferric citrate and peptidoglycan(a TLR-2 ligand)were unable to alter the in vitro expression of hepcidin in HT-29 ***:Colitis increased local hepcidin-25 expression,which was associated with the IL-6/Stat-3 signaling *** increase in local iron sequestration was also