The anti-inflammatory regulation of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
作者机构：Department of Ophthalmologythe Affiliated Hospital of Qingdao UniversityQingdao 266003Shandong ProvinceChina
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2020年第13卷第5期
页 面：701-707页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金：Supported by National Natural Science Foundation of China(No.81870632) the Youth National Natural Science Foundation of China(No.81800800 No.81700800) the Natural Science Foundation of Shandong Province(No.ZR2017BH025)
主 题：calcitonin gene-related peptide fungal keratitis NLRP3 mice
摘 要：AIM:To analyze the impact of calcitonin gene-related peptide(CGRP)in mouse keratitis after Aspergillus fumigatus(***)***:C57 BL/6 mice were treated subconjunctivally with different concentrations of exogenous CGRP,and BALB/c mice were treated with CGRP8-37(a CGRP antagonist)before corneas were infected with *** cornea was assessed under the slit-lamp and the clinical score was *** mRNA levels of IL-1β,TNF-α,IL-6,and MIP-2 were detected by quantitative real-time polymerase chain reaction(PCR),while the protein level of IL-1βwas determined by Western *** vitro,RAW264.7 cells were used to investigate NLRP3 and IL-1βexpression induced by *** after the pretreatment of exogenous CGRP or *** expression in RAW264.7 cells was evaluated by real-time PCR and Western ***:Using exogenous CGRP resulted in downregulated synthesis of IL-1βand MIP-2 stimulated by *** in C57 BL/6 mice keratitis,and the synthesis of IL-1β,MIP-2 and IL-6 was up-regulated in BALB/c mice corneas after the pretreatment with *** with exogenous CGRP and CGRP8-37 did not influence TNF-αmRNA levels either in BALB/c or C57 BL/6 mice *** levels of NLRP3 and IL-1βwere both reduced in *** stimulated-macrophages after treatment with exogenous *** CGRP8-37 pretreatment would increase NLRP3 and IL-1β***:CGRP may alleviate the inflammatory reaction in mice keratitis after infection with *** anti-inflammatory effect may be related to the inhibition of NLRP3 expression by CGRP.
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