Effects of low level laser treatment on the survival of axotomized retinal ganglion cells in adult Hamsters

作     者：Kwok-Fai So Mason Chin Pang Leung Qi Cui 

作者机构：GHM Institute of CNS Regenerationand Guangdong Key Laboratory of Brain Function and DiseasesJinan University Department of AnatomyLKS Faculty of MedicineThe University of Hong Kong Department of OphthalmologyLKS Faculty of MedicineThe University of Hong Kong Department of Rehabilitation SciencesThe Hong Kong Polytechnic University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第21期

页      面：1863-1869页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：supported by Key Basic Research Project of China(973 Program),No.2011CB707501 Fundamental Research Funds for The Central Universities,No.21609101 

主　　题：low level laser treatment retinal ganglion cells optic nerve injury neuroprotection mi-croglial proliferation optic nerve axotomy 

摘      要：Injury to axons close to the neuronal bodies in the mammalian central nervous system causes a large proportion of parenting neurons to degenerate. It is known that optic nerve transection close to the eye in rodents leads to a loss of about half of retinal ganglion cells in 1 week and about 90% in 2 weeks. Using low level laser treatment in the present study, we demonstrated that treatment with helium-neon （660 nm） laser with 15 mW power could delay retinal ganglion cell death after optic nerve axotomy in adult hamsters. The effect was most apparent in the ifrst week with a short period of treatment time （5 minutes） in which 65–66% of retinal ganglion cells survived the optic nerve axotomy whereas 45–47% of retinal ganglion cells did so in optic nerve axotomy controls. We also found that single dose and early commencement of laser irradiation were important in protecting retinal ganglion cells following optic nerve axotomy. These ifndings thus convincingly show that appropriate laser treatment may be neuroprotective to retinal gan-glion cells.