Isolation and Characterization of a Cinnamoyl CoA Reductase Gene(CCR) in Pear(Pyrus pyrifolia)

作     者：Meng HU Boya TAN Tao WU Xianming LI Junfan TU Fuchen YANG Hongyan ZHU Zhongqi QIN 

作者机构：College of Life SciencesWuhan University Institute of Fruit and TeaHubei Academy of Agricultural Sciences Hubei Innovation Center of Agricultural Science and Technology 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2014年第15卷第6期

页      面：926-932页

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：Supported by the Earmarked Fund for China Agriculture Research System(CARS-2917) Hubei Innovation Center of Agricultural Science and Technology(2011-620-005003) 

主　　题：Pear(Pyrus pyrifolia) Stone cell Lignin Cinnamoyl-CoA-reductase 

摘      要：Pear is a popular and commercially important fresh fruit, and its texture is related to the presence of sclereid formatted by parenchyma cell with lignification in vascular plants. Previous studies have demonstrated that content of lignin may be regulated by cinnamoyl CoA reductase(CCR) in various plants. However, the function of CCR in pears remains very limited. In the present study, we isolated a cDNA encoding CCR(PpCCR, GenBank accession No. KF999958) and its promoter(proPpCCR) from Whangkeumbae pear to investigate the function of CCR in lignin biosynthesis. PpCCR-GFP expressed in rice mesophyll protoplast demonstrated that PpCCR-GFP was localized in the cytoplasm, indicating that CCR may function in cytoplasm without localization signals. In transgenic plants carrying PpCCR, we observed higher lignin content compared with that in wild type plants, further suggesting that PpCCR can affect the lignin contents through regulating lignin biosynthesis in Arabidopsis thaliana. More studies in other plants are needed to confirm our conclusion.