Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells

作     者：Junping Yao Wensheng Hou Hao Wang Hui Liu Chuanhuang Weng Zhengqin Yin 

作者机构：College of BioengineeringChongqing UniversityChongqing 400044China Chongqing Key Lab of Visual Damage and Regeneration&RestorationChongqing 400038China Department of OphthalmologySouthwest HospitalThird Military Medical University of Chinese PLAChongqing 400038China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第16期

页      面：1228-1233页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 071002[理学-动物学] 

基　　金：sponsored by the National Natural Science Foundation of China (General Program),No.81070749,31070882,30970758 Key Project of Chongqing Science & Technology Commission,No. 2010AB5118 

主　　题：channelrhodopsin-2 recombinant adenovirus visual cortex blue light action potentials neuralregene ration 

摘      要：Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells and 24 hours later, cells were transfected with recombinant adenovirus carrying channelrhodopsin-2 and green fluorescent protein genes. After 2 4 days of transfection, green fluorescence was visible in the cultured cells. Cells were stimulated with blue light （470 nm）, and light-induced action potentials were recorded in patch-clamp experiments. Our findings indicate that channelrhodopsin-2-recombinant adenovirus transfection of primary cultured visual cortical cells can control the production of action potentials via blue light stimulation.