Effect of PKC pathway on G_1/S progression control in HeLa cells

作     者：HU Jiang & LIU HuituDepartment of Biology, Beijing Normal University, Beijing 100080, China Correspondence should be addressed to Liu Huitu 

作者机构：Department of Biology Beijing Normal University Beijing China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2000年第45卷第5期

页      面：440-442页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：中国科学院资助项目(34930080) 

主　　题：PKC cell cycle early response gene engine molecules. 

摘      要：The effect of PKC activity on G1/S progression in HeLa cells has been studied. The result shows that (i) PKC activity alteration in G1 phase affects d/S progression in HeLa cells. it has been observed that G1/S progression is stimulated by PKC agonist TPA and inhibited by PKC inhibitor GF-109203X. (ii) The expression of c-myc and c-jun is stimulated by TPA and inhibited by GF-109203X treatment in early G1 phase. (iii) During G1/S progression, the expression of CyciinD1is stimulated by TPA treatment and inhibited by GF-109203X treatment. There is no effect on the expression of CDK4. it is likely that PKC pathway regulates G1/S progression through regulating the expression of some early response genes and engine molecules in HeLa cells.