Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo
作者机构：Department of Electronic and Computer EngineeringThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Division of Life ScienceThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Department of Ophthalmology and Visual SciencesThe Chinese University of Hong KongHong KongChina State Key Laboratory of Molecular NeuroscienceThe Hong Kong University of Science and TechnologyClear Water BayKowloonHongKongChina Center of Systems Biology and Human HealthThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2020年第9卷第1期
页 面：1298-1308页
核心收录：
学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学]
基 金：supported by the Hong Kong Research Grants Council through grants 662513,16103215,16148816,16102518,16149316,T13-607/12R,T13-706/11-1,AOE/M-09/12,T13-605/18W,C6002-17GF,C6001-19EF and N_HKUST603/19 the Hong Kong University of Science and Technology(HKUST)through grant RPC10EG33 the Innovation and Technology Commission through grant ITCPD/17-9 the Health and Medical Research Fund through grant HMRF18SC17
主 题：optics functional photon
摘 要：In vivo fundus imaging offers non-invasive access to neuron structures and biochemical processes in the ***,optical aberrations of the eye degrade the imaging resolution and prevent visualization of subcellular retinal *** developed an adaptive optics two-photon excitation fluorescence microscopy(AO-TPEFM)system to correct ocular aberrations based on a nonlinear fluorescent guide star and achieved subcellular resolution for in vivo fluorescence imaging of the mouse *** accurate wavefront sensing and rapid aberration correction,AOTPEFM permits structural and functional imaging of the mouse retina with submicron ***,simultaneous functional calcium imaging of neuronal somas and dendrites was ***,the timelapse morphological alteration and dynamics of microglia were characterized in a mouse model of retinal *** addition,precise laser axotomy was achieved,and degeneration of retinal nerve fibres was *** highresolution AO-TPEFM is a promising tool for non-invasive retinal imaging and can facilitate the understanding of a variety of eye diseases as well as neurodegenerative disorders in the central nervous system.
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