Increased CD133^+ cell infiltration in the rat brain following fluid percussion injury

作     者：Ming Wei Ziwei Zhou Shenghui Li Chengwei Jing Dashi Zhi Jianning Zhang 

作者机构：Tianjin Neurological Institute General Hospital Tianjin Medical University Tianjin 300052 China Department of Neurosurgery Second Hospital of Tianjin Medical University Tianjin 300070 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第4期

页      面：278-282页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 080704[工学-流体机械及工程] 08[工学] 080401[工学-精密仪器及机械] 0807[工学-动力工程及工程热物理] 071006[理学-神经生物学] 0804[工学-仪器科学与技术] 

基　　金：supported by the National Natural Science Foundation of China, No. 30772229 the National Basic Research Program of China (973 Program), No. 2005CB522600 the Science Foundation of Tianjin Bureau of Public Health, No. 2011KZ96 

主　　题：prominin-1 immunohistochemistry reverse transcription-PCR traumatic brain injury neural regeneration 

摘      要：The prominin-1/CD133 epitope is expressed in craniocerebral trauma in animal models of fluid undifferentiated cells. Studies have reported that percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133＋ cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133＋ cells were mainly distributed in hippocampal CA1 3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.