Genomics functional analysis and drug screening of SARS-CoV-2

作     者：Long Chen Li Zhong 

作者机构：Bioengineering Institute of Chongqing University174 Shazheng StreetChongqingChina 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2020年第7卷第4期

页      面：542-550页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：We thank Dr.Zhang for providing the genome sequence of SARS-CoV-2.We should also thank Yuqing Chen Minglu Niu and Luyao Zhao for reference providing and relevant analysis of the virus and potential drugs.This study cannot go well without the help of these experts 

主　　题：SARS-CoV-2 Drug screening Epitope Genomic Homology miRNA ORF 

摘      要：A novel coronavirus appeared in Wuhan,China has led to major ***,rapid classification of virus species,analysis of genome and screening for effective drugs are the most important *** the present study,through literature review,sequence alignment,ORF identification,motif recognition,secondary and tertiary structure prediction,the whole genome of SARS-CoV-2 were comprehensively *** find effective drugs,the parameters of binding sites were calculated by *** addition,potential miRNAs were predicted according to RNA *** prediction by using NCBI,WebMGA and Gene-Mark and comparison,a total of 8 credible ORFs were *** the whole genome have great difference with other CoVs,each ORF has high homology with SARS-CoVs(90%).Furthermore,domain composition in each ORFs was also similar to *** the DrugBank database,only 7 potential drugs were screened based on the sequence search *** predicted binding sites between drug and ORFs revealed that 2-(N-Morpholino)-ethanesulfonic acid could bind 1#ORF in 4 different regions ***,both benzyl(2-oxopropyl)carbamate and 4-(dimehylamina)benzoic acid have bene demonstrated to inhibit SARS-CoV infection ***,2 miRNAs(miR-1307-3p and miR-3613-5p)were predicted to prevent virus replication via targeting 30-UTR of the genome or as *** conclusion,the novel coronavirus may have consanguinity with *** used to treat SARS may also be effective against the novel *** addition,altering miRNA expression may become a potential therapeutic schedule.