LINC00511 promotes gastric cancer cell growth by acting as a ceRNA

作     者：Chong-Bing Sun Hong-Yi Wang Xiao-Qing Han Yong-Ning Liu Meng-Chun Wang Hong-Xia Zhang You-Feng Gu Xiao-Gang Leng 

作者机构：Department of General SurgeryWeifang People's HospitalWeifang 261041Shandong ProvinceChina Department of Anorectal SurgeryWeifang People's HospitalWeifang 261041Shandong ProvinceChina Department of Spine SurgeryWeifang People's HospitalWeifang 261041Shandong ProvinceChina 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第4期

页      面：394-404页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：LINC00511 miR-124-3p PDK4 Long noncoding RNAs Gastric cancer 

摘      要：BACKGROUND Gastric cancer(GC)is one of the most aggressive malignancies,with a high incidence and poor prognosis ***,accumulating evidence has illustrated that long noncoding RNAs(lnc RNAs)play pivotal roles in many *** has been reported that LINC00511 contributes to tumorigenesis in various ***,the role of LINC00511 in GC cell growth remains mostly *** To determine whether the lnc RNA LINC00511 exerted its carcinogenic function in GC via the miR-124-3 p/PDK4 *** Cell culture and transfection,RNA extraction and quantitative real-time PCR,CCK-8 assay,Colony formation assay,Luciferase reporter assay,RIP assay,RNA pull-down assay,and Western blot analysis were used to show expression and mechanisms of LINC00511 in GC progression and *** assays were performed to verify the relationships among LINC00511,miR-124-3 p and PDK4 *** The expression of LINC00511 was remarkably upregulated in GC cells compared to that in corresponding normal cell *** to the controls,cell proliferation was inhibited,and cell apoptosis was increased upon LINC00511knockdown,demonstrating that LINC00511 influenced GC cell *** exploration of the molecular mechanism revealed that LINC00511 functioned as a molecular sponge of miR-124-3 p and that PDK4 was a downstream target of miR-124-3 p in *** assays showed that the overexpression of PDK4 could partly restore the inhibitory function of si-LINC00511 in *** These data demonstrate that LINC00511 promotes gastric cancer cell growth by acting as a ce RNA to regulate the miR-124-3 p/PDK4 axis,which may be a promising therapeutic target for GC.