Association mapping for root system architecture traits under two nitrogen conditions in germplasm enhancement of maize doubled haploid lines

作     者：Langlang Ma Chunyan Qing Ursula Frei Yaou Shen Thomas Lübberstedt Langlang Ma;Chunyan Qing;Ursula Frei;Yaou Shen;Thomas Lübberstedt

作者机构：Department of AgronomyIowa State UniversityAmes 50010USA Key Laboratory of Biology and Genetic Improvement of Maize in Southwest RegionMaize Research InstituteSichuan Agricultural UniversityChengdu 611130China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2020年第8卷第2期

页      面：213-226页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：China Scholarship Council(CSC)for Langlang Ma’s funding USDA’s National Institute of Food and Agriculture(IOW04314,IOW01018) Hatch Multistate Project NC-007 the R.F.Baker Center for Plant Breeding at Iowa State University,for supporting this work. 

主　　题：germplasm breeding seedling 

摘      要：Root system architecture(RSA)contributes to nitrogen(N)uptake and utilization in maize.In this study,a germplasm enhancement of maize double haploid population of 226 lines genotyped with 61,634 SNPs was used to investigate the genetic basis of RSA under two N levels using a genome-wide association study(GWAS).GLM+PCA,FarmCPU,and MLM models were utilized to balance false positives and false negatives.In total,33 and 51 significant SNP-trait associations were detected under high and low N conditions,respectively.Under high N,SNP S9_2483543 was detected by all models.Linkage disequilibrium(LD)regions of some SNPs overlapped with the intervals of QTL for RSA and N response that were detected in previous studies.In particular,several known genes,Rtcs,Rtcl,Rtcl,and Ms44,were located in the LD regions of S1_9992325,S9_151726472,S9_154381179,and S4_197073985,respectively.Among the candidate genes identified by this study,GRMZM2G139811,GRMZM2G314898,GRMZM2G054050,GRMZM2G173682,GRMZM2G470914,GRMZM2G462325,GRMZM2G416184,and GRMZM2G064302 were involved in seedling,seed,and root system development or N metabolism in Arabidopsis or rice.The markers identified in this study can be used for marker-assisted selection of RSA traits to improve nitrogen use efficiency in maize breeding,and the candidate genes will contribute to further understanding of the genetic basis of RSA under diverse N conditions.