Soil organic carbon and phosphorus availability regulate abundance of culturable phosphate-solubilizing bacteria in paddy fields

作     者：Arvind KUMAR Lal Chand RAI Arvind KUMAR;Lal Chand RAI

作者机构：Molecular Biology SectionLaboratory of Algal BiologyCenter of Advanced Study in BotanyBanaras Hindu UniversityVaranasi 221005India Department of BiotechnologyGuru Ghasidas VishwavidyalayaBilaspur 495009India 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2020年第30卷第3期

页      面：405-413页

核心收录：

学科分类：07[理学] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：support through a network project on microbial diversity the Department of Science and Technology,India 

主　　题：bacterial abundance insoluble P organic acids phosphate solubilization phylogenetic analysis 16S rRNA gene 

摘      要：Low availability of phosphorus(P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore,phosphate-solubilizing bacteria(PSB) from paddy field soils of the Indo-Gangetic Plain, India were isolated, and their abundance was attempted to be correlated with the physicochemical characteristics of the soils. Ninety-four PSB were isolated on Pikovskaya’s agar medium, and quantitative phosphate solubilization was evaluated using NBRIP medium. The isolates solubilized P up to a concentration of 1 006 μg mL-1 from tricalcium phosphate with the secretion of organic acids. These isolates were identified by 16 S rRNA gene sequence comparison, and they belonged to Gammaproteobacteria(56 isolates),Firmicutes(28 isolates), Actinobacteria(8 isolates), and Alphaproteobacteria(2 isolates). Phylogenetic analysis confirmed the identification by clustering the isolates in the clade of the respective reference organisms. The correlation analysis between PSB abundance and physicochemical characteristics revealed that the PSB population increased with increasing levels of soil organic carbon, insoluble P, K+, and Mg2+. The promising PSB explored in this study can be further evaluated for their biofertilizer potential in the field and for their use as potent bio-inoculants.